In our lab, mass spectrometry is an essential part of preclinical immunology and in vitro research for drug discovery and development. The technique can provide in-depth information about the regulation of the immune system and the molecular mechanisms involved in immune responses. Mass spectrometry-based proteomics is used in immunology research to study protein expression, (sub)cellular localization, secretion, protein modifications, and interactions in immune cells upon activation by different stimuli.

Immundnz develops and applies bioanalytical methods for use in immune proteomics and biomarker analysis in preclinical research. Available techniques are mass spectrometry (LC-MS) and immunoassays (ELISA). We employ these techniques in combination with multivariate analysis for biomarker identification such as proteomics and biomarker quantification and validation studies, especially in immunology and infection.

IMMUNOPROTEOMICS

Immundnz is your provider of immunoproteomics services, the study of proteins involved in the immune response. Mass spectrometry in combination with multivariate analysis is used to identify proteins that are modulated by a specific  drug, disease or infection in comparison to a control group. Other applications of immunoproteomics include the identification of potential protein targets of antibodies and monitor purification by these antibodies and the characterization of MHC binding peptides. Inflammasomes are critical for the clearance of pathogens and damaged cells but, if uncontrollable, can also be a driver of autoimmune disease. Using a proteomics approach, components of the innate immune system that regulate inflammasomes can be identified and studied.

BIOMARKER ANALYSIS

The quantitative analysis of protein biomarkers is performed to study e.g. immune response as a result of disease- or drug-induced tissue damage, secondary pathology and immunogenicity. We have absolute quantification assays for RAGE and TLR2/4 receptor proteins. These can be used e.g. to study the release of intracellular proteins to the extracellular matrix in in vitro studies.