The Immune Response Company
Immunology CRO specialising in experimental immunology, immune response and tissue damage analysis
Oncology, immuno-oncology and systemic diseases such as cardiovascular, respiratory, metabolic, and autoimmune conditions and infections are areas of therapeutic development that require advanced immunology understanding, immune-based solutions and translational data.
Immundnz is an immunology CRO with customised services and in-depth knowledge in immune mechanisms and protein biology to provide you with the solutions you need to make better decisions in drug discovery and at the non-clinical and pre-clinical stage.
We are scientists who enjoy hiking and research. We go off the beaten track and specialise in designing, developing and experimenting with pre-clinical models (in vitro and ex vivo) to study immune interactions.
Immune Modelling & Experimental Design
We specialise in designing, developing and experimenting with pre- and non-clinical models (in vitro, ex vivo and in vivo) to study immune interactions.
Immune modelling is the design of an experimental model to test the immune response associated with an infection, a metabolite, or a drug. Studies in experimental immunology commonly involve complex immune pathways that cannot be tested by single or standard assays. The designing of the experiment in a model is necessary with the consideration and knowledge of multiple factors in the signalling cascade, the interaction of multiple cells and their immunogenic or tolerogenic response.
Inflammation & Systemic Conditions
Tissue Damage &
Vaccines, Drug Delivery & Transfection
We are ready to support you at any point in your trail in drug research and development
Customised Assays for Tailored Solutions
Every drug in development targets a unique site or function for which every problem is unique and requires a solution that is generated through its assessment. We are experienced scientists who will customise an assay or assays to provide a tailored solution with the understanding of the celluar and signalling pathway that is involved. These assays are immunologic, protein-based and bioanalytical based on primary cells and/or cell lines.
Some of our popular assays:
- M1-M2 Polarisation Assays
- Macrophage Phagocytosis (ADCP)
- Cytokine Release Assay (CRA)
- Mixed Lymphocyte Reaction (MLR)
- Dendritic Cell Maturation and Activation
- T Cell Proliferation
- T Cell Differentiation
- Cell Stress & Apoptosis
- Cell Viability & Cytotoxicity
- Immune & Protein-based Assays
Macrophages are important myeloid cells of the immune system that are phagocytic and secrete cytokines that are instrumental in promoting a pro-inflammatory or regulatory immune response and interact with other immune effectors. The M1 (pro-inflammatory) and M2 (regulatory) are the two main macrophage types that are considered important in drug biology studies. Read more.
Phagocytosis is an immunologic mechanism involved in apoptosis, necrosis, infection and tumour conditions mediated by leukocytes like macrophages, DCs, and neutrophils.
One of the types of phagocytosis is antibody-dependent cellular phagocytosis (ADCP). In this case, antibody-opsonised target cells activate phagocytes via Fc-FcR binding, leading to internalisation and degradation of the target cell. This mechanism is valuable in infection and tumour biology and the basis of many antibody-based drugs. Read more.
Cytokines are small proteins that allow cells of the immune system to communicate with each other. When the Immune System produces these cytokines uncontrollably in response to a biopharmaceutical molecule, for instance, or a viral antigen, a cytokine release syndrome or “cytokine storm” is triggered. As a result, high levels of inflammation are created, which can be fatal. Read more.
Mixed Lymphocyte Reaction (MLR) is an in vitro assay in which immune cells from two different donors are co-cultured together to trigger an immune response. MLR can be performed in different ways. In one-way MLR, T cells from one donor are mixed with antigen-presenting cells from another donor. In two-way MLR, two PBMC populations are combined, each containing both T cells and antigen-presenting cells. The activation of T cells within both populations can be measured simultaneously. The advantage of MLR is that no stimulation with a stimulatory compound is required to induce an immune response. T cells and antigen-presenting cells can be analysed by flow cytometry and/or immune-assays for cytokines, activation markers or intracellular protein expression. MLR is often used as to determine the immunomodulatory potential of a compound, especially their ability to potentiate a T cell response. Read more.
Dendritic cells (DCs) are the star antigen-presenting cells (APCs) of myeloid or haematopoietic origin that play a vital role between the innate and adaptive immune systems.
DCs are pivotal in the T-cell-mediated T-helper response to the MHC ClassII-Ag complex and cross-presenting via the MHC class I pathway. They consist of receptors for pathogen and danger-associated molecular patterns, e.g. TLRs. Read more.
An in vitro T cell proliferation assay can be used to determine whether or not T cells are triggered to divide after exposure to a specific antigen of interest, or to assess differences between cell populations in their ability to divide in response to an antigen. We use flow cytometry to assess markers for T cell proliferation. The T cell proliferation assay can be used to assess modulation of the T cell response by (immunomodulatory) compounds. Read more.
Our in-house human T cell assay is adapted to differentiate Th17 cells from TALL-1 cells. On stimulation (e.g. with PMA), these cells produce IL-17 and IL-22, quantified by intracellular flow cytometry or ELISA. This assay can be performed in 96-well plates to test the effect of compounds on Th17 differentiation and activation (e.g. inhibitory effect) and the viability of Th17 cells. The assay can be also be performed with primary T cells. Read more.
Cell apoptosis is also known as programmed cell death, and is a highly regulated process that allows for proper growth and development by destroying unneeded cells and tissues. An apoptosis assay can detect and quantify the cellular events associated with programmed cell death. We can assess apoptosis and cellular stress in a variety of ways, using flow cytometry, Western blot, microscopy or fluorometric. Read more.
Cell viability assays are used to determine if a test molecule has effects on cell proliferation or if it shows cytotoxic effects that lead to cell death. Cell viability assays can also be used to determine optimal growth conditions in cell culture studies. We can assess cell viability in a variety of ways, using flow cytometry, immune assays, microscopy or colorimetry. Read more.
We perform various immunologic and protein-based assays that are needed in your research or drug development. While we provide standard assays our speciality is in developing custom designed assays suited for your product. Read more.
Learn how Immundnz guides immunologic research
To study the role of heat shock proteins in immune-mediated cell death and autoimmunity, we designed a diabetes model consisting of transgenic expression of the target protein. We initiated cell death of islet beta-cells in a controlled dose-dependent manner. The study successfully revealed that the (i) initial beta cell death was responsible for initiating immune-mediated diabetes, and (ii) the immune response was increased by the overexpression of heat shock protein. This study was essential to understand the role of heat shock proteins in immunogenicity following tissue damage.
Let’s go for a hike. Together.
Our experts are ready to set up a route and be on your way to understand which solution or assay as a path will help you to reach from one point to another in your drug research.
We’d be happy to discuss and help you in your research.