We are a specialist service provider focused in immunology and cell cultures for your needs in preclinical drug development. We specialise in the characterisation of cells and cell fluid (culture supernatant).
We use various cellular, immunologic and molecular methods to draw a comprehensive analysis of drug-induced immunogenicity. The methods are also provided as general or customised service for routine or specific analysis.
You will need an immune model if developing an immunotherapeutic that is expected to function through generating an immune response (e.g. against tumours) or if the drug targets an immune condition (e.g. autoimmunity) or if the drug might generate an adverse immune response.
We emphasise on the immune modelling of a disease or a candidate therapeutic to understand:
- what is the immune profile of the disease and why is it important?
- what is the immune response to the candidate biomolecule, and is it important?
We are consultants in designing the model for immune response in in vitro and in vivo and we will carry out laboratory experiments (in vivo and/or in vitro) to test the model.
We perform services that are needed in your immunology research, or research that requires immunologic methods. We provide these services either as standard assays or as custom designed assays to meet your requirement.
We have a range of methods and techniques available, such as cell cultures, microscopy, flow cytometry, fluorescence and absorbance reading (microplates), Western blotting, immunohistochemistry and also mass spectrometry. We use these for:
- Cell characterization (surface and intracellular expression)
- Cell culture fluid analysis for proteins, metabolites, cytokines and other biomarkers
- Cell lysate analysis
- In vitro immune assays
- Cell death analysis
- Antibody applications testing
- Tissue damage assessment
Cell Culture Systems
Cell culture systems are at the heart of our expertise and services. We grow human and mammalian cells in various culture media, both adherent and non-adherent (e.g. lymphocytes) type. We grow various established and primary human cells in our lab that represent human tissue systems such as:
- Aortic smooth muscle cells (SMCs)
- Lung epithelial cells (A549)
- Embryonic renal cells (HEK293)
- Hepatic cells (HepG2)
- Cervical cancer cells (HeLa)
- Placental cells (BeWo)
- Retinal epithelial cells (ARPE19)
- Neuronal cells (SY5Y)
- Prostate cancer cells (PC3)
- Lymphocytic cells (THP1, TALL1, BALL1)
- Monocyte derived dendritic cells
We can grow other cells to meet your needs and customise an in vitro human cell study.
We use various human tissue systems to study:
- Drug treatment impact on protein expression
- Cell to cell interaction
- Migration of cells
- Cell attachment and spreading
- Cell death and cytotoxicity
- Functional assay using gene knockdown
- Mixed Lymphocyte Reaction (MLR) Assays
- Antigen specific T cell activation Assays
- Macrophage polarization assays
- T cell proliferation
- Cytokine secretion profile
- Cell surface marker expression analysis
Tissue Damage Assessment
The endogenous molecules or proteins that are released by cells through drug-induced necrosis or compromised membrane are activators of an immune response. By characterising these molecules in cells and cell fluids in combination with pathway analysis, we are able to assess drug-induced tissue damage. This is a very important step in drug risk assessment at preclinical stage.
COMPIT, Comprehensive In Vitro Human Immune Response Testing of drug compounds, is our major product to study the interaction of a drug compound or biomolecule with tissues and the extent of damage that is caused by this interaction to understand drug induced adverse immune response. COMPIT can be applied to small molecule drugs, large molecule drugs, generics and biosimilars.