We are a service provider focused around immunology and cell cultures. We specialise in the characterisation of cells, cell fluids and culture supernatants.
We use various cellular, immunologic and molecular methods to draw a comprehensive analysis of drug-induced immunogenicity. The methods are also provided as general or customised service for routine or specific analysis.
We perform services that are needed in your immunology research, or research that requires immunologic methods. We provide these services either as standard assays or as custom designed assays to meet your requirement.
We have a range of methods and techniques available, such as cell cultures, microscopy, flow cytometry, fluorescence and absorbance reading, western blotting, immunohistochemistry and also mass spectrometry. We use these for:
- Cell characterization – surface and intracellular expression
- Cell culture fluid analysis for proteins, metabolites, cytokines and other biomarkers
- Cell lysate analysis
- In vitro immune assays
- Cell death analysis
- Antibody applications testing
- Tissue damage assessment
Cell Culture Systems
Cell culture systems are at the heart of our expertise and services. We grow human and mammalian cells in various culture media, both adherent and non-adherent (e.g. lymphocytes) type. We grow established (cell lines) and primary cells in 2D cultures as well as in 3D tissue models. These in vitro systems are used by us to investigate biological features and drug compound interactions.
Some frequently used cell types in our laboratory are HEK293, HeLa, HepG2, Thp-1 (monocytic), human aortic SMC, fibroblasts, dendritic cells, T cells and B cells.
Tissue Damage Assessment
The endogenous molecules or proteins that are released by cells through drug-induced necrosis or compromised membrane are activators of an immune response. By characterising these molecules in cells and cell fluids in combination with pathway analysis and sophisticated bio-informatics, we are able to assess drug-induced tissue damage.
COMPIT, Comprehensive In Vitro Human Immune Response Testing of drug compounds, is our main product to study the interaction of a drug compound or biomolecule with tissues and the extent of damage that is caused by this interaction to understand drug induced adverse immune response. COMPIT can be applied to small molecule drugs, large molecule drugs, generics and biosimilars.