ASSAYS

IMMUNE & PROTEIN BASED

We perform various immunologic and protein based assays that are needed in your research or drug development. While we provide standard assays our speciality is in developing custom designed assays suited for your product.

Cell Line Based Approach

We use lymphocytc cell lines for various immune response assays such as dendritic cell maturation, T cell activation, phagocytosis, etc. We are among the few groups who can generate and demonstrate the use of dendritic cells from monocytic cell lines and T cells in activation studies. Conventionally dendritic cells are generated from primary monocytes or bone marrow and T cells or monocytes are separated from primary PBMCs from human donors. The use of cell lines in the initial stage offers a consistent reference assay prior to using primary human cells. Primary human cells used in the initial stage can be variable, expensive, time consuming, and often inconclusive.

STANDARD

CUSTOMISED

IN VITRO

EX VIVO

METHODS

  • Co-cultures, co-culture in a chip/chamber
  • Microscopy
  • Flow cytometry
  • ELISA
  • Fluorescence and absorbance reading
  • Western blotting
  • Immunohistochemistry
  • Mass spectrometry (small and large molecules)
  • RNA interference (siRNA)
  • Protein expression/ Transfection
  • Surface plasmon resonance (SPR)
  • Subcellular fractionation
  • RNA extraction and quantitation

We use the methods for:

  • Cell characterization (surface and intracellular marker expression)
  • Cell culture fluid analysis for proteins, metabolites, cytokines and other biomarkers
  • Cell lysate analysis
  • In vitro immune assays
  • Cell death analysis
  • Tissue damage assessment
  • Protein functional study
  • Antibody applications testing

If you would like to know more about these assays or about other assays in immunology or protein biology that we can develop for you please Contact Us.

ASSAYS

  • Dendritic cell maturation assay
  • Th differentiation assay
  • T cell activation assay
  • Cytokine secretion assay
  • Mixed lymphocyte assay
  • Antigen specific T cell activation assay
  • Cell death assay/ induced tissue damage
  • Functional assay (gene knockdown and knock-in)
  • Adhesion assay
  • MTT assay
  • LDH assay
  • Cleaved caspase assay
  • Scratch assay (wound healing)

Please contact for more information.

APPLICATIONS (Immunology)

  • Th differentiation
  • Dendritic cell maturation
  • T cell activation
  • Mixed Lymphocyte Reaction (MLR)
  • Antigen specific T cell activation
  • Macrophage polarization
  • Cytokine secretion profile

Please contact to discuss what may be relevant to your work and what we can develop to suit your product. Much of our work is customised assay development.

APPLICATIONS (Cell & Protein)

  • Drug treatment impact on protein expression
  • Cell to cell interaction
  • Migration of cells
  • Cell attachment and spreading
  • Cell proliferation
  • Cell death and cytotoxicity
  • Cell surface marker expression analysis
  • Protein function (transfection or RNA)
  • Protein expression and localisation
  • Wound healing
In vivo and Ex vivo

We are highly experienced in murine in vivo work, bovine tissue explantation work and ex vivo assays. We collaborate with the Alderley Park in vivo facility. We can develop assays based on these systems within our premises to fit the purpose of your project. Please contact for more information.

Image, left: immunofluorescence staining of mouse pancreatic tissue